abstract：:The matrix metalloproteinase matrilysin (MMP-7) is a member of the matrix metalloproteinase gene family, which is believed to play an important role in tumor invasion and metastasis. We have previously found that matrilysin mRNA is specifically expressed in colorectal cancers and adenomas and that its message is local...

journal_title：Journal of clinical laboratory analysis

authors： Yamamoto H,Itoh F,Senota A,Adachi Y,Yoshimoto M,Endoh T,Hinoda Y,Yachi A,Imai K

更新日期：1995-01-01 00:00:00

abstract：:As several possible prognostic and therapeutic applications of interleukin-6 are currently under trial, the available methods for its quantification in biological fluids should be evaluated. In this report, the 7TD1 hybridoma bioassay is compared to an enzyme immunoassay for the determination of interleukin-6 in serum...

journal_title：Journal of clinical laboratory analysis

authors： Papadopoulos NG,Dedoussis GV,Baxevanis CN,Papamichail M

更新日期：1995-01-01 00:00:00

abstract：:We evaluated the Miles Inc., Clinitek Atlas Automated Urine Chemistry Analyzer for 11 tests: bilirubin, color, glucose, ketones, leukocyte esterase, nitrite, occult blood, pH, protein, specific gravity, and urobilinogen. The instrument uses a roll of reagent strips affixed to a clear plastic support; urine specimens a...

journal_title：Journal of clinical laboratory analysis

authors： Lott JA,Johnson WR,Luke KE

更新日期：1995-01-01 00:00:00

abstract：:The incidence of aneuploidy was determined by flow cytometric analysis in paraffin-embedded tumor samples of 125 patients with non-Hodgkin lymphoma (NHL). There were 48 low-grade (LG) and 77 high-grade (HG) tumors. Aneuploidy was found in 34 (27%) NHL, 15 (31%) LG, and 19 (25%) HG tumors. The analysis of patient survi...

journal_title：Journal of clinical laboratory analysis

authors： Vucković J,Forenpoher G,Marusić M,Uzarević B,Sabioncello A,Stula N,Dubravcić M

更新日期：1995-01-01 00:00:00

abstract：:Determination of free digoxin levels in patients treated with digoxin immune FAb has long been a problematic area in clinical laboratory testing. The older radioimmunoassays resulted in inaccurate and variable results due to the competition of the administered drug with the radioactively labelled forms. The 1995 Physi...

journal_title：Journal of clinical laboratory analysis

authors： Honda SA,Rios CN,Murakami L,Morita T,Scottolini AG,Bhagavan NV

更新日期：1995-01-01 00:00:00

abstract：:In this study, the effect of the synthetic glucocorticoid hormone dexamethasone (DXM) on HSV replication was studied in a DXM receptor-positive mouse neuroblastoma (NB) cell line. In cells treated with 10(-7) M DXM and then infected with HSV, there was a statistically significant 9-18-fold increase in the amount of vi...

journal_title：Journal of clinical laboratory analysis

authors： Sawiris GP,Sydiskis RJ,Bashirelahi N

更新日期：1994-01-01 00:00:00

abstract：:The distribution of the major VL subgroups (V lambda I, V lambda II, V lambda III, V lambda IV, V lambda VI, and V lambda VIII) among lambda-type immunoglobulins (Igs) in normal serum was determined by a sandwich enzyme-linked immunosorbent assay (ELISA) using a panel of murine anti-human V lambda-subgroup-specific mo...

journal_title：Journal of clinical laboratory analysis

authors： Abe M,Ozaki S,Wolfenbarger D,deBram-Hart M,Weiss DT,Solomon A

更新日期：1994-01-01 00:00:00

abstract：:The human insulin domains, signal peptide, B-chain, C-peptide, and A-chain, were highly expressed in Escherichia coli as recombinant proteins N-terminally fused to glutathione-S-transferase and a histidine-hexapeptide. The recombinant proteins were purified from insoluble cell fraction by affinity chromatography using...

journal_title：Journal of clinical laboratory analysis

authors： Walter M,Berg H,Northemann W

更新日期：1994-01-01 00:00:00

abstract：:A simple, rapid assay for the pancreatic isoenzyme of human serum amylase was developed. The assay utilized an immunoabsorbent prepared by coating latex beads with a monoclonal antibody specific for pancreatic amylase. Treatment of patient serum with immunoabsorbent removed pancreatic amylase, and measurement of resid...

journal_title：Journal of clinical laboratory analysis

authors： Landt M,Hortin GL,Smith CH,Pashos G,Vaidya HC,Rosenblum JL

更新日期：1994-01-01 00:00:00

abstract：:An automated measurement of total and free hydroxyproline in serum or urine is presented that uses flow injection analysis. After exclusion of nonspecific substances, hydroxyproline was oxidized by chloramine-T and L-cysteine with Ehrlich's reagent. The linearity obtained was from 3.8 mumole/L to 1.22 mmole/L with goo...

journal_title：Journal of clinical laboratory analysis

authors： Uji Y,Karmen A,Okabe H,Hata K,Miura M,Ozaki K,Minamizaki M,Shibata T,Inayama S

更新日期：1994-01-01 00:00:00

abstract：:During the past few years, interest in xenotransplantation of porcine islets of Langerhans for the future therapy of type I diabetes has increased markedly. Therefore, we established a semiautomated digestion method for isolating islets from the porcine pancreas. However, although the isolation technique was standardi...

journal_title：Journal of clinical laboratory analysis

authors： Heiser A,Ulrichs K,Müller-Ruchholtz W

更新日期：1994-01-01 00:00:00

abstract：:We studied a new proteinase K assay method for human serum mitochondrial aspartate aminotransferase. We found that proteinase K showed no inactivation of human mitochondrial aspartate aminotransferase isoenzyme and complete inactivation of cytosolic aspartate aminotransferase. Previous studies have shown that selectiv...

journal_title：Journal of clinical laboratory analysis

authors： Watazu Y,Uji Y,Okabe H,Shirahase Y,Kaneda N,Karmen A

更新日期：1993-01-01 00:00:00

abstract：:We found that the Rhabdomyosarcoma (RD) cell line expresses human acetylcholine receptor (AChR) based on the following evidences: 1. Soluble AChR can be isolated from RD cells following the isolation procedure for AChR from human muscle; 2. Intact RD cells bind to alpha-bungarotoxin (alpha Butx) in a time-dependent an...

journal_title：Journal of clinical laboratory analysis

authors： Wu JT,Astill M,Lloyd C,Salmon VC

更新日期：1993-01-01 00:00:00

abstract：:The newly developed immunoblot assay, RIBA SIA (recombinant and synthetic polypeptide immunoblot assay), Chiron, Calif., was compared with the commercially available second generation recombinant immunoblot assay (RIBA-2) for the detection of antibody to hepatitis C virus (anti-HCV). The two immunoblot tests were also...

journal_title：Journal of clinical laboratory analysis

authors： Chaudhary RK,Andonov A,MacLean C

更新日期：1993-01-01 00:00:00

abstract：:Using a fully automated reticulocyte counter, the roles of the reticulocyte count with maturation in pancytopenia were evaluated. Different groups of pancytopenia including aplastic anemia, infiltrative marrow disorder, hypersplenism, and megaloblastic anemia were recruited. All patients had bone marrow examinations f...

journal_title：Journal of clinical laboratory analysis

authors： Lin CK,Hsu HC,Chau WK,Jiang ML,Chiu CF

更新日期：1993-01-01 00:00:00

abstract：:Ninety semen samples from male patients were studied. They were classified into 4 different groups; normospermic fertile, oligospermia, asthenospermia, and oligoasthenospermia. In addition to routine semen analysis, the hypo-osmotic swelling test was done for all these samples, measuring total protein and specific gra...

journal_title：Journal of clinical laboratory analysis

authors： al-Mogazy HM,al-Khadra S,Kenawy G,al-Zeftawy A,Mostafa T,Attia A,Attia AM

更新日期：1993-01-01 00:00:00

abstract：:This study compared the use of conventional urine microscopy, the KOVA system, Neubauer hemacytometry, and the Yellow IRIS for the determination of white blood cell (WBC) and red blood cell (RBC) counts in urine samples. Both KOVA WBC and RBC counts correlated better with the IRIS counts than with conventional microsc...

journal_title：Journal of clinical laboratory analysis

authors： McGinley M,Wong LL,McBride JH,Rodgerson DO

更新日期：1992-01-01 00:00:00

abstract：:An immunoenzymometric assay (IEMA) for a new CA125-like antigen, CA602, was developed. Five monoclonal antibodies raised against a human ovarian carcinoma cell line could detect their respective antigens in the sera of ovarian carcinoma patients. The antigen levels detected in serum by the various antibodies correlate...

journal_title：Journal of clinical laboratory analysis

authors： Mochizuki H,Sato I,Nagoya K,Furusako S,Yamauchi T,Masuko H,Sato H,Morishita H,Yajima M,Sasaki H

更新日期：1992-01-01 00:00:00

abstract：:Polyfunctional hydrophilic microspheres of 125-nm diameter can be produced by copolymerization of acrylic monomers. Purified c-reactive protein (CRP) was covalently bound to these new micropheres, and the conjugate obtained was used as reagent in a microparticle-enhanced nephelometric immunoassay for human CRP. This a...

journal_title：Journal of clinical laboratory analysis

authors： Montagne P,Laroche P,Cuillière ML,Varcin P,Pau B,Duheille J

更新日期：1992-01-01 00:00:00

abstract：:Didemnin A was conjugated at the amino terminus of the N-methylleucine residue, via the linkers N-succinimidyl-3-(2-pyridyldithio)-propionate and trans-1,4-maleimidomethyl-cyclohexane carboxylic acid, to keyhole limpet hemocyanin (KLH) and bovine serum albumin (BSA). The didemnin-KLH conjugates were used to hyperimmun...

journal_title：Journal of clinical laboratory analysis

authors： Raybould TJ,Grothaus PG,Simpson SB,Bignami GS,Lazo CB,Newman RA

更新日期：1992-01-01 00:00:00

abstract：:A novel and ultrasensitive noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay) for alpha-human atrial natriuretic peptide (alpha-hANP) in plasma is described. alpha-hANP was biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-alpha-hANP [6-28] IgG-coated polystyre...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Ishikawa E

更新日期：1992-01-01 00:00:00

abstract：:A sensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for (anti-human T-cell leukemia virus type I) IgG (anti-HTLV-I IgG) in serum using recombinant gag p24(14-214) of HTLV-I is described. The recombinant gag p24(14-214) is soluble in the absence of detergents and allows the use of enzymes other ...

journal_title：Journal of clinical laboratory analysis

authors： Kohno T,Hirota K,Sakoda I,Yamasaki M,Yokoo Y,Ishikawa E

更新日期：1992-01-01 00:00:00

abstract：:Novel molecular approaches have recently become available allowing improved major histocompatibility complex (MHC) matching of potential allogeneic bone marrow donors and recipients. Current cellular and serological assays are hindered by aberrant cell populations and limited reagents which only detect an individuals'...

journal_title：Journal of clinical laboratory analysis

authors： Rubocki RJ,Wisecarver JL,Hook DD,Cox SM,Beisel KW

更新日期：1992-01-01 00:00:00

abstract：:A modification of the assay system for peroxidase activity in human mixed saliva using 2,2'-azinobis-(-ethylbenzthiazoline-6-sulphonic acid) (ABTS) as the substrate is reported. The modifications permit differentiation between glandular peroxidase (SPX) and myeloperoxidase (MPX) activity. In addition, factors endogeno...

journal_title：Journal of clinical laboratory analysis

authors： Venezie RD,Jenzano JW,Lundblad RL

更新日期：1991-01-01 00:00:00

abstract：:Using a specific serum anti-soluble T lymphocytes receptor for sheep erythrocytes (E) and SDS-PAGE, we detected radioactive bands of molecular weight 58,000 in immunoprecipitates of supernatant of heated human lymphocytes (SHL), in the supernatant of PHA stimulated lymphocyte cultures (SLC), normal human serum (NHS), ...

journal_title：Journal of clinical laboratory analysis

authors： Itano EN,Ono MA,Sumigawa M,Longo IM,Moura NC,Mendes NF

更新日期：1991-01-01 00:00:00

abstract：:Cholesterol in the diet can readily autoxidize and be absorbed and transported in plasma lipoproteins. Cholesterol oxides can also be endogenously produced in tissues via free-radical-induced reactions. Some cholesterol oxides, notably cholestane-3 beta, 5 alpha, 6 beta-triol and 25-hydroxycholesterol, have been shown...

journal_title：Journal of clinical laboratory analysis

authors： Peng SK,Hu B,Morin RJ

更新日期：1991-01-01 00:00:00

abstract：:We evaluated the Technicon DPA-1 immunoassay analyzer on its analytical characteristics. Therefore we studied three assays: albumin in cerebrospinal fluid and IgG and transferrin in serum. When tested with the Cusum test for linearity albumin, IgG, and transferrin measurements showed no deviation from linearity. Close...

journal_title：Journal of clinical laboratory analysis

authors： van Rijn JL,van Landeghem AA,Goldschmidt HM

更新日期：1991-01-01 00:00:00

abstract：:A novel and sensitive noncompetitive enzyme immunoassay (hetero-two-site enzyme immunoassay) for alpha-human atrial natriuretic peptide (alpha-hANP) in plasma, which uses only one monoclonal IgG for the ring structure of alpha-hANP, is described. Plasma was filtered through polysaccharide membrane to separate peptides...

journal_title：Journal of clinical laboratory analysis

authors： Hashida S,Yamamoto N,Ishikawa E

更新日期：1991-01-01 00:00:00

abstract：:We have evaluated a new latex nephelometric test for the quantitation of myoglobin in human serum. The assay consists of incubating the diluted serum sample (20-fold) for 12 min at room temperature with latex particles covalently coated with anti-myoglobin antibodies and then quantifying the change of light-scatter pr...

journal_title：Journal of clinical laboratory analysis

authors： Borque L,Rus A,Ruiz R

更新日期：1991-01-01 00:00:00

abstract：:A radioactive polymerase chain reaction (PCR) method has been developed for the relative quantification of the human alpha-2 chain of type I collagen [hu alpha-2(I)] in cells. cDNAs generated by reverse transcription from the total pool of cytoplasmic RNA serve as a template for polymerase chain reaction amplification...

journal_title：Journal of clinical laboratory analysis

authors： Yu YL,Golden JA,Migchielsen AA,Goetzl EJ,Turck CW

更新日期：1991-01-01 00:00:00

abstract：:The direct agglutination test (DAT) and the enzyme-linked immunosorbent assay (ELISA) were used for serodiagnosis of parasitologically confirmed Indian visceral leishmaniasis (VL) cases. All the sera of VL cases were positive by both the methods. DAT titres of VL cases were greater than or equal to 1:3,200, and ELISA ...

journal_title：Journal of clinical laboratory analysis

authors： Pal A,Mukerji K,Basu D,Naskar K,Mallick KK,Ghosh DK

更新日期：1991-01-01 00:00:00

abstract：:A sensitive time-resolved fluorimetric immune-complex-transfer immunoassay for antithyroglobulin IgG in serum is described. Antithyroglobulin IgG in test serum was reacted with dinitrophenyl europium ion-labeled thyroglobulin. The complex formed of antithyroglobulin IgG and dinitrophenyl europium ion-labeled thyroglob...

journal_title：Journal of clinical laboratory analysis

authors： Kohno T,Mitsukawa T,Matsukura S,Ishikawa E

更新日期：1990-01-01 00:00:00

abstract：:We studied antibody production in serial serum samples from patients with acute Epstein-Barr virus (EBV) and cytomegalovirus (CMV) infections. Sera were analyzed both by enzyme-linked immunosorbent assay (ELISA) using a synthetic peptide (P62) derived from the glycine-alanine repeating region of the Epstein-Barr nucle...

journal_title：Journal of clinical laboratory analysis

authors： Rhodes G,Smith RS,Rubin RE,Vaughan J,Horwitz CA

更新日期：1990-01-01 00:00:00

abstract：:The effects of TPA (12-0-tetradecanoylphorbol-13-acetate) and RA (retinoic acid) were investigated on the cell lines HL60 (acute promyelocytic leukemia) and K562 (erythroleukemia) and on cells from patients with several kinds of leukemia. There were 14 cases of acute lymphocytic leukemia (ALL), 2 cases of chronic lymp...

journal_title：Journal of clinical laboratory analysis

authors： Marques-Silva VM,De Souza MH,Teixeira MC,Arcuri RA,Rumjanek VM

更新日期：1990-01-01 00:00:00

abstract：:Acid phosphatase was purified to electrophoretic homogeneity from human normal lung and spleen and was characterized biochemically and immunologically in comparison with prostate acid phosphatase (PAP). The apparent MW of lung acid phosphatase (LAP) and spleen acid phosphatase (SAP) was 110,000 and 100,000, respective...

journal_title：Journal of clinical laboratory analysis

authors： Lin MF,Li SS,Chu TM,Lee CL

更新日期：1990-01-01 00:00:00

abstract：:An enzyme-linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum-absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum-absorbed te...

journal_title：Journal of clinical laboratory analysis

authors： Thiele GM,Rogers J,Collins M,Yasuda N,Smith D,McDonald TL

更新日期：1990-01-01 00:00:00

abstract：:In 23 patients with common migraine (CM), immune responsiveness and frequency of immunocompetent cells were investigated. In particular, phagocytosis and killing of Candida albicans by polymorphs (PMNs) and monocytes were analyzed. Also, the percentages of CD3+, CD4+, CD8+, natural killer, and CD15+ cells were evaluat...

journal_title：Journal of clinical laboratory analysis

authors： Covelli V,Maffione AB,Munno I,Jirillo E

更新日期：1990-01-01 00:00:00

abstract：:A flow cytometric method for quantitation of glutathione (GSH) was applied to simultaneous analysis of the major leukocyte types in peripheral blood. Cellular thiols (predominantly GSH) were stained with monochlorobimane (MCIB), and thiol fluorescence was measured with a flow cytometer. The fluorescence of the thiols ...

journal_title：Journal of clinical laboratory analysis

authors： Scott RB,Collins JM,Matin S,White F,Swerdlow PS

更新日期：1990-01-01 00:00:00

abstract：:We studied 103 patients seen in our Prostate Cancer Detection Clinic to determine whether a correlation exists between serum prostate-specific antigen (PSA) values and ultrasound-calculated prostate gland volume. Seventy men (68%) had a PSA value less than or equal to 4 ng/ml (our upper limit of normal). The men were ...

journal_title：Journal of clinical laboratory analysis

authors： Babaian RJ,Fritsche HA,Evans RB

更新日期：1990-01-01 00:00:00

abstract：:Graphical representation of laboratory data speeds up recognition of abnormalities. The Chernoff face is an excellent tool to represent patient data; the human eye and mind are exquisitely sensitive to subtle changes in facial appearances and expression. One of the best uses of the faces is in illustrating trends in t...

journal_title：Journal of clinical laboratory analysis

authors： Lott JA,Durbridge TC

更新日期：1990-01-01 00:00:00